Cytogenetic study on antlions (Neuroptera, Myrmeleontidae): first data on telomere structure and rDNA location.

Myrmeleontidae, commonly known as "antlions", are the most diverse family of the insect order Neuroptera, with over 1700 described species (in 191 genera) of which 37 species (in 21 genera) have so far been studied in respect to standard karyotypes. In the present paper we provide first data on the occurrence of the "insect-type" telomeric repeat (TTAGG) and location of 18S rDNA clusters in the antlion karyotypes studied using fluorescence hybridization (FISH). We show that males of (Linnaeus, 1764) (Palparinae), (Villers, 1789) (Acanthaclisinae) and (Fabricius, 1798) (Nemoleontinae) have rDNA clusters on a large bivalent, two last species having an additional rDNA cluster on one of the sex chromosomes, most probably the X. (TTAGG) - containing telomeres are clearly characteristic of and ; the presence of this telomeric motif in is questionable. In addition, we detected the presence of the (TTAGG) telomeric repeat in (Scopoli, 1763) from the family Ascalaphidae (owlflies), a sister group to the Myrmeleontidae. We presume that the "insect" motif (TTAGG) was present in a common ancestor of the families Ascalaphidae and Myrmeleontidae within the neuropteran suborder Myrmeleontiformia.