AI Article Synopsis
- The study introduces a new method using MYC and CDKN1A mRNA imaging to identify G1-phase cycle arrest in cancer cells.
- The imaging technique produces a visual change from green to red, indicating the decrease of MYC and increase of CDKN1A mRNA levels.
- Researchers applied this imaging on cancer cells treated with TMPyP4 to investigate the involvement of HIF-1α in hindering cell proliferation.
Article Abstract
The identification of G1-phase arrest requires a dynamic capturing method. In this work, we reported an indicator based on MYC and CDKN1A mRNA imaging to visualize G1-phase cycle arrest. A typical G1-phase arrest imaging is shown as a green-to-red conversion by fluorescence molecular beacons indicating the change as down-regulating of MYC and up-regulating of CDKN1A mRNA. We performed G1-phase arrest imaging on proliferated cancer cells inhibited by TMPyP4 and explore the role of HIF-1α in the proliferation inhibition effect.
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| http://dx.doi.org/10.1039/c6cc10078c | DOI Listing |
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