5-Hydroxymethylcytidine (hmC) was recently identified as a direct metabolite of mC in RNA. We investigated the stability of hmC in human cells using bio-isotopologues and LC-MS/HRMS. This has led to the discovery of a second oxidative metabolite of mC in RNA, namely 2'-O-methyl-5-hydroxymethylcytidine (hmCm). Subsequent quantitative analysis of total RNA from higher organisms revealed varying levels and TET-independent formation of this new RNA modification.
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| http://dx.doi.org/10.1021/jacs.6b12180 | DOI Listing |
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