We reported that coronary spasm was induced in the transgenic mice with the increased phospholipase C (PLC)-δ1 activity. We investigated the effect of enhanced PLC-δ1 on Ca influx and its underlying mechanisms. We used human embryonic kidney (HEK)-293 and coronary arteries smooth muscle cells (CASMC). Intracellular free Ca concentration ([Ca ] ; nm) was measured by fura-2, and Ca influx was evaluated by the increase in [Ca ] after addition of extracellular Ca . Acetylcholine (ACh) was used to induce Ca influx. ACh-induced peak Ca influx was 19 ± 3 in control HEK-293 cells and 71 ± 8 in the cells with PLC-δ1 overexpression (P < 0.05 between two groups). Nifedipine partially suppressed this Ca influx, whereas either 2-APB or knockdown of classical transient receptor potential channel 6 (TRPC6) blocked this Ca influx. In the human CASMC, ACh-induced peak Ca influx was 29 ± 6 in the control and was increased to 45 ± 16 by PLC-δ1 overexpression (P < 0.05). Like HEK-293 cells, pretreatment with nifedipine partially suppressed Ca influx, whereas either 2-APB or knockdown of TRPC6 blocked it. ACh-induced Ca influx was enhanced by PLC-δ1 overexpression, and was blocked partially by nifedipine and completely by 2-APB. TRPC-mediated Ca influx may be related to the enhanced Ca influx in PLC-δ1 overexpression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/fcp.12269 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Flotillins in membrane trafficking and physiopathology.
Biol Cell
January 2025
CRBM (Centre de Recherche en Biologie cellulaire de Montpellier), BIOLuM, University of Montpellier, CNRS UMR 5237, Montpellier, France.
Flotillin 1 and 2 are highly conserved and homologous members of the stomatin, prohibitin, flotillin, HflK/C (SPFH) family. These ubiquitous proteins assemble into hetero-oligomers at the cytoplasmic membrane in sphingolipid-enriched domains. Flotillins play crucial roles in various cellular processes, likely by concentrating sphingosine.
View Article and Find Full Text PDFE74-like ETS transcription factor 3 expression and regulation in human intervertebral disc.
JOR Spine
March 2025
SERGAS (Servizo Galego de Saude) and IDIS (Instituto de Investigación Sanitaria de Santiago), The NEIRID Group (Neuroendocrine Interactions in Rheumatology and Inflammatory Diseases) Santiago University Clinical Hospital Santiago de Compostela Spain.
Background: Intervertebral disc degeneration (IVDD) is one of the main causes of chronic low back pain. The degenerative process is often initiated by an imbalance between catabolic and anabolic pathways. Despite the large socio-economic impact, the initiation and progress of disc degeneration are poorly understood.
View Article and Find Full Text PDFCitrus transcription factor CsERF1 is involved in the response to citrus tristeza disease.
Front Plant Sci
January 2025
National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University/Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University, Chongqing, China.
Introduction: Citrus tristeza virus (CTV) is a threat to the citrus production and causes severe economic losses to the citrus industry. Ethylene response factors (ERFs) play important roles in plant growth and stress responses. Although ERF genes have been widely studied in model plants, little is known about their role in biological stress responses in fruit trees, such as citrus.
View Article and Find Full Text PDFtranscription factor plays a role in regulating anthocyanin biosynthesis in pear () skin.
Front Plant Sci
January 2025
College of Horticulture, Sichuan Agricultural University, Chengdu, China.
The phenylacetone pathway, which encompasses flavonoids, lignin, and other compounds, is of paramount importance in determining the quality of pear fruit. Nevertheless, the precise regulatory functions of R2R3-MYB transcription factors in the metabolic pathways that regulate pear color changes remain unclear. In this study, we isolated an transcription factor from 'Red Zaosu' pears and demonstrated that it influenced the expression of several genes, including , and .
View Article and Find Full Text PDFLysyl oxidase-like 2 promotes the survival, migration, and ferroptosis of endometrial cancer cells by activating the phosphoinositide 3-kinase/protein kinase B pathway.
Iran J Basic Med Sci
January 2025
Department of Obstetrics and Gynecology, Shanghai Pudong Hospital of Fudan University, Pudong, Shanghai-201399, China.
Objectives: LOXL2, known as Lysyl oxidase-like 2, is classified as a lysyl oxidase (LOX) family member. However, its role and mechanism in endometrial cancer (EC) are unknown. Therefore, we aimed to investigate the potential role and mechanism of LOXL2 in EC.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!