Premise Of The Study: Microsatellite loci were isolated from four species of (Adoxaceae) to study population structure and assess species boundaries among morphologically similar South American species of the clade.
Methods And Results: Using a microsatellite-enriched library and mining next-generation sequence data, 16 microsatellites were developed. Each locus was tested on two populations of and one population of . For nuclear loci, one to 13 alleles were recovered, expected heterozygosity ranged from 0 to 0.8975, Simpson diversity index ranged from 0.0167 to 1.000, and Shannon diversity index ranged from 0 to 2.3670 in a given population. For the mitochondrial locus, three to six alleles were recovered and unbiased haploid diversity values ranged from 0.756 to 0.853 in a given population.
Conclusions: The 16 microsatellite loci developed for the clade (, Adoxaceae) will inform investigations of population structure and species boundaries within this group.
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| http://dx.doi.org/10.3732/apps.1600103 | DOI Listing |
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