An expanded nuclear phylogenomic PCR toolkit for Sapindales.

Premise Of The Study: We tested PCR amplification of 91 low-copy nuclear gene loci in taxa from Sapindales using primers developed for (Burseraceae).

Methods And Results: Cross-amplification of these markers among 10 taxa tested was related to their phylogenetic distance from . On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16-90). (Brassicales), by contrast, yielded product for two. Single representatives of Anacardiaceae and Rutacaeae yielded 34 and 26 products, respectively. Twenty-six primer pairs worked for all Burseraceae species tested if highly divergent is excluded, and eight of these amplified product in every Sapindalean taxon.

Conclusions: Our study demonstrates that customized primers for can amplify product in a range of Sapindalean taxa. This collection of primer pairs, therefore, is a valuable addition to the toolkit for nuclear phylogenomic analyses of Sapindales and warrants further investigation.