AI Article Synopsis
- - Protein complexes are essential for many biological processes, but understanding their detailed functions can be difficult.
- - The review highlights the importance of mass spectrometry in studying protein assemblies and their roles in cellular pathways, particularly in the context of the ubiquitin-proteasome system responsible for protein degradation.
- - It encourages a combined use of different mass spectrometry techniques to gain deeper insights into complex biological systems.
Article Abstract
Protein complexes form the critical foundation for a wide range of biological process, however understanding the intricate details of their activities is often challenging. In this review we describe how mass spectrometry plays a key role in the analysis of protein assemblies and the cellular pathways which they are involved in. Specifically, we discuss how the versatility of mass spectrometric approaches provides unprecedented information on multiple levels. We demonstrate this on the ubiquitin-proteasome proteolytic pathway, a process that is responsible for protein turnover. We follow the various steps of this degradation route and illustrate the different mass spectrometry workflows that were applied for elucidating molecular information. Overall, this review aims to stimulate the integrated use of multiple mass spectrometry approaches for analyzing complex biological systems.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5238952 | PMC |
| http://dx.doi.org/10.1017/S0033583516000160 | DOI Listing |
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