AI Article Synopsis
- The rapid aminoglycoside NP test quickly identifies multiple aminoglycoside resistance by detecting glucose metabolism in enterobacteria when exposed to amikacin and gentamicin, indicated by a color change in a pH indicator.
- It was tested on various bacterial isolates, including 18 resistant strains with 16S rRNA methylases and 20 with AG-modifying enzymes, along with 10 susceptible isolates.
- The test showed high accuracy with 100% sensitivity and 97% specificity when compared to the traditional broth dilution method, and it is affordable and can be used globally within less than two hours.
Article Abstract
The rapid aminoglycoside NP (Nordmann/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance in It is based on the detection of the glucose metabolism related to enterobacterial growth in the presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The rapid aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG-resistant isolates producing 16S rRNA methylases, 20 AG-resistant isolates expressing AG-modifying enzymes (acetyl-, adenyl-, and phosphotransferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97%, respectively, compared to the broth dilution method, which was taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (<2 h), and implementable worldwide.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5377834 | PMC |
| http://dx.doi.org/10.1128/JCM.02107-16 | DOI Listing |
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