AI Article Synopsis
- This study investigates the role of abnormal DNA methylation in the development of pre-B acute lymphoblastic leukemia (ALL) by analyzing both DNA methylation and gene expression data.
- Researchers identified specific intergenic regions with altered methylation patterns that correlate with changes in gene expression in pre-B ALL patients, with most of these regions also being associated with key transcription factors involved in B-cell development.
- A significant decrease in enhancer RNA (eRNA) levels was observed, linked to the downregulation of important genes related to B-cell migration, growth, and cell death, underscoring the potential of targeting these regulatory areas for new therapies in pre-B ALL.
Article Abstract
A complete understanding of the mechanisms involved in the development of pre-B ALL is lacking. In this study, we integrated DNA methylation data and gene expression data to elucidate the impact of aberrant intergenic DNA methylation on gene expression in pre-B ALL. We found a subset of differentially methylated intergenic loci that were associated with altered gene expression in pre-B ALL patients. Notably, 84% of these regions were also bound by transcription factors (TF) known to play roles in differentiation and B-cell development in a lymphoblastoid cell line. Further, an overall downregulation of eRNA transcripts was observed in pre-B ALL patients and these transcripts were associated with the downregulation of putative target genes involved in B-cell migration, proliferation, and apoptosis. The identification of novel putative regulatory regions highlights the significance of intergenic DNA sequences and may contribute to the identification of new therapeutic targets for the treatment of pre-B ALL.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478429 | PMC |
| http://dx.doi.org/10.1080/10428194.2016.1272683 | DOI Listing |
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