AI Article Synopsis
- The study focuses on the role of long noncoding RNAs (lncRNAs) in regulating acute lymphoblastic leukemia (ALL) by examining differences in methylation patterns of antisense lncRNAs in leukemia versus normal pre-B-cell lymphoblasts.
- Researchers used a methylated CpG island recovery assay along with next-generation sequencing (NGS) to analyze the methylome of ALL and normal lymphoblasts.
- The findings suggest that AS-lncRNA may trans-regulate 368 genes with high sequence similarity, indicating its significant role in biological processes and potentially offering new approaches for classifying and treating ALL.
Article Abstract
Aim: Long noncoding RNAs serve critical regulatory functions highly specific for a tissue and its developmental stage. Antisense long ncRNA (AS-lncRNA) methylation changes in acute lymphoblastic leukemia (ALL) versus normal pre-B-cell lymphoblasts were evaluated to identify potential differential methylation in this group of genes.
Materials & Methods: The methylome of ALL and normal lymphoblasts was examined by the methylated CpG island recovery assay followed by NGS.
Conclusion: The potential effect of trans regulation by AS-lncRNA through DNA/RNA binding is significant as sequence alignment analysis of the 25 most differentially methylated AS-lncRNAs revealed 368 genes containing highly similar sequences with a median nucleotide identity of 90.8% and binding span of 122 base pairs. Regulation of biological processes and anatomical structure development were over represented. ALL classification schemes based on AS-lncRNA methylation can provide new insights into its pathogenesis and treatment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040088 | PMC |
| http://dx.doi.org/10.2217/epi-2016-0156 | DOI Listing |
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