AI Article Synopsis
- Tryptophan synthase is an enzyme with complex allosteric interactions that regulate its two subunits' activity and ligand gate functions.
- The enzyme carries out 13 different reaction steps, transferring intermediate products directly between its subunits.
- A stochastic model developed in previous research is utilized to analyze the thermodynamics of tryptophan synthase, exploring the Gibbs energy landscape, entropy production, and information exchange due to allosteric interactions.
Article Abstract
The enzyme tryptophan synthase is characterized by a complex pattern of allosteric interactions that regulate the catalytic activity of its two subunits and opening or closing of their ligand gates. As a single macromolecule, it implements 13 different reaction steps, with an intermediate product directly channeled from one subunit to another. Based on experimental data, a stochastic model for the operation of tryptophan synthase has been earlier constructed [D. Loutchko, D. Gonze, and A. S. Mikhailov, J. Phys. Chem. B 120, 2179 (2016)]. Here, this model is used to consider stochastic thermodynamics of such a chemical nanomachine. The Gibbs energy landscape of the internal molecular states is determined, the production of entropy and its flow within the enzyme are analyzed, and the information exchange between the subunits resulting from allosteric cross-regulations and channeling is discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1063/1.4973544 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative transcriptome profiling reveals the mechanism of increasing lysine and tryptophan content through pyramiding , and genes in maize.
Breed Sci
September 2024
Guizhou Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou Province, China.
To explore the molecular mechanism behind maize grain quality and use of different gene stacking to improve the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing , along with the double-recessive mutant lines containing , , and . The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (, , and ) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis.
View Article and Find Full Text PDFUnraveling the interactive effect of opaque2 and waxy1 genes on kernel nutritional qualities and physical properties in maize (Zea mays L.).
Sci Rep
January 2025
ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
The mutant waxy allele (wx1) is responsible for increased amylopectin in maize starch, with a wide range of food and industrial applications. The amino acid profile of waxy maize resembles normal maize, making it particularly deficient in lysine and tryptophan. Therefore, the present study explored the combined effects of genes governing carbohydrate and protein composition on nutritional profile and kernel physical properties by crossing Quality Protein Maize (QPM) (o2o2/wx1wx1) and waxy (o2o2/wx1wx1) parents.
View Article and Find Full Text PDFIncrease in IAA levels by EPSPS copy number variation relates to fitness advantage in Eleusine indica.
Pest Manag Sci
January 2025
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Background: Long-term use of chemical weed control has led to some weedy species evolving herbicide resistance traits with fitness advantage. Our previous studies revealed glyphosate resistance in an Eleusine indica population due to copy number variation of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) comes with fitness advantage under non-competitive conditions. Here, transcriptomics and targeted metabolomics were used to investigate physiological basis associated with the fitness advantage.
View Article and Find Full Text PDFBiological studies reveal the role of trpA gene in biofilm formation, motility, hemolysis and virulence in Vibrio anguillarum.
Microb Pathog
January 2025
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Marine Biomedical Science and Technology Innovation Platform of Lin-gang Special Area, Shanghai 201306, China. Electronic address:
Vibrio anguillarum is a pathogen responsible for vibriosis in aquaculture animals. The formation of bacterial biofilm contributes to infections and increases resistance to antibiotics. Tryptophanase and its substrate tryptophan have been recognized as signal molecules regulating bacterial biofilm formation.
View Article and Find Full Text PDFRegulation of anaplerotic enzymes by melatonin enhances resilience to cadmium toxicity in Vigna radiata (L.) R. Wilczek.
Plant Physiol Biochem
January 2025
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Melatonin (Mel) is a tryptophan-derived (N-acetyl-5-methoxytryptamine) molecule. In the present study, role of Mel in the regulation of various anaplerotic enzymes is discussed in relation to N metabolism and H-ATPase activity in mung bean under Cd stress. The application of Mel to the Cd-stressed mung bean seedlings was remarkable in improving the activity of hexokinase (35.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!