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Engineering a Novel NIR RNA-Specific Probe for Tracking Stress Granule Dynamics in Living Cells.
Anal Chem
January 2025
Institute of Physical Science and Information Technology, Information Materials and Intelligent Sensing Laboratory of Anhui Province, Key Laboratory of Structure and Functional Regulation of Hybrid Materials of Ministry of Education, Anhui University, Hefei, Anhui 230601, China.
Real-time monitoring of the dynamics of cytosolic RNA-protein condensates, termed stress granules (SGs), is vital for understanding their biological roles in stress response and related disease treatment but is challenging due to the lack of simple and accurate methods. Compared with protein visualization that requires complex transfection procedures, direct RNA labeling offers an ideal alternative for tracking SG dynamics in living cells. Here, we propose a novel molecular design strategy to construct a near-infrared RNA-specific fluorescent probe () for tracking SGs in living cells.
View Article and Find Full Text PDFTeratological abnormalities in hard ticks (Acari: Ixodidae) from Pakistan.
Exp Appl Acarol
December 2024
Department of Zoology, Abdul Wali Khan University Mardan, Mardan, 23200, Pakistan.
Studying teratological abnormalities in ticks are taxonomically important because this poorly understood biological phenomenon causes difficulties in tick's identification. Globally, reports regarding these abnormalities in ticks, reasons of their causes and their impacts are scarce. According to the available published data, there are no studies regarding teratological abnormalities in ticks from Pakistan.
View Article and Find Full Text PDFPotential causes of iatrogenic intraoperative bleeding during C1 surgeries: a CT 3D rendering study.
Spine J
November 2024
Department of Radiology, China-Japan Union Hospital of Jilin University, Changchun, China. Electronic address:
Article Synopsis
- Iatrogenic intraoperative bleeding during C1 surgeries is challenging to control, prompting a study to investigate its possible causes.
- The study analyzed high-resolution CTA images from 551 patients to evaluate variations in the vertebral artery and polymorphisms of ponticulus posticus, along with venous plexus communications.
- Findings indicated that anatomical variations, particularly regarding POPOs and venous structures, can contribute to bleeding; a preoperative CTA is suggested to improve surgical outcomes and reduce risks.*
Design and fabrication of superhydrophobic microstructured grooved substrates to suppress the coffee-ring effect and enhance the stability of Sr element detection in liquids using LIBS.
Anal Methods
December 2024
School of Quality and Technical Supervision, Hebei University, Baoding, 071002, China.
A new technique has been developed to enhance the stability of laser-induced breakdown spectroscopy (LIBS) in the analysis of dry droplets by mitigating the coffee ring effect (CRE) on substrates with superhydrophobic microstructured grooves. The substrate was prepared from a laser-etched pure copper base, resembling the surface of a lotus leaf, creating a biomimetic superhydrophobic substrate. The superhydrophobic microstructured grooved substrate contained an array of dome-shaped cones with heights of approximately 140 μm and 100 μm, arranged in a periodic pattern of high-low-high.
View Article and Find Full Text PDFIs chemodenervation with incobotulinumtoxinA an alternative to invasive chronic anal fissure treatments?
BMC Gastroenterol
September 2024
Servicio de Cirugía General y Aparato Digestivo, Hospital Nuestra Señora del Prado. Talavera de La Reina, Toledo, Spain.
Article Synopsis
- Botulinum toxin type A (incoBoNT/A) is effective in treating anal fissures (AFs) that haven't responded to other treatments, showing a healing rate of 83.9% after 2 years in a study with 49 patients.
- The treatment involved injecting the toxin into specific areas around the anal sphincter, resulting in significant reductions in anal resting pressure and pain perception over time, although there was a temporary increase in incontinence that resolved.
- The study suggests incoBoNT/A offers a safe, less invasive alternative to surgery for AF treatment, especially for patients unresponsive to ointment therapy, with only mild and temporary adverse events reported.
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