Paenibacillus polymyxa WLY78 is a nitrogen fixer and it can be potentially applied to biofertilizer in agriculture. In this study, P. polymyxa WLY78 is labelled with gfp gene. The GFP-labelled P. polymyxa WLY78 is used to inoculate wheat, maize and cucumber seedlings grown in the gnotobiotic system and in soil, respectively. Observation by confocal laser scanning microscope reveals that the GFP-labeled bacterial cells are mainly located on the root surface and epidermis of wheat, and only a few cells are present within cortical cells. In maize and cucumber seedlings, bacterial cells were colonized in epidermal and cortical cells, intercellular spaces and vascular system of root, stem and leaf tissue interiors besides on root surfaces. Higher densities of the bacterial cells in roots, stems and leaves indicated that P. polymyxa WLY78 cells could migrate from roots to stems and leaves of maize and cucumber. This study will provide insight into interaction between P. polymyxa WLY78 and host cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5226731 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169980 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
Structure modification of an antibiotic: by engineering the fusaricidin bio-synthetase A in .
Front Microbiol
September 2023
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Fusaricidin, a lipopeptide antibiotic, is specifically produced by strains, which could strongly inhibit fungi. Fusaricidin bio-synthetase A (FusA) is composed of six modules and is essential for synthesizing the peptide moiety of fusaricidin. In this study, we confirmed the FusA of strain WLY78 involved in producing Fusaricidin LI-F07a.
View Article and Find Full Text PDFGlutamine synthetase and GlnR regulate nitrogen metabolism in WLY78.
Appl Environ Microbiol
September 2023
Key Laboratory of Soil Microbiology of Agriculture Ministry and College of Biological Sciences, China Agricultural University, Beijing, China.
WLY78, a N-fixing bacterium, has great potential use as a biofertilizer in agriculture. Recently, we have revealed that GlnR positively and negatively regulates the transcription of the (trogen ixation) operon () in WLY78 by binding to two loci of the promoter according to nitrogen availability. However, the regulatory mechanisms of nitrogen metabolism mediated by GlnR in the genus remain unclear.
View Article and Find Full Text PDFGenome-wide mapping of GlnR-binding sites reveals the global regulatory role of GlnR in controlling the metabolism of nitrogen and carbon in Paenibacillus polymyxa WLY78.
BMC Genomics
February 2023
State Key Laboratory for Agrobiotechnology, College of Biological Sciences and Key Laboratory of Soil Microbiology of Agriculture Ministry, China Agricultural University, Beijing, People's Republic of China.
Background: Paenibacillus polymyxa WLY78 is a Gram-positive, endospore-forming and N-fixing bacterium. Our previous study has demonstrated that GlnR acts as both an activator and a repressor to regulate the transcription of the nif (nitrogen fixation) operon (nifBHDKENXhesAnifV) according to nitrogen availability, which is achieved by binding to the two GlnR-binding sites located in the nif promoter region. However, further study on the GlnR-mediated global regulation in this bacterium is still needed.
View Article and Find Full Text PDFFusaricidin Biosynthesis Is Controlled via a KinB-Spo0A-AbrB Signal Pathway in WLY78.
Mol Plant Microbe Interact
December 2021
State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing, China.
Fusaricidins produced by are important lipopeptide antibiotics against fungi. The (fusaricidin biosynthesis) operon is responsible for synthesis of fusaricidins. However, the regulation mechanisms of fusaricidin biosynthesis remain to be fully clarified.
View Article and Find Full Text PDFIdentification of Genes Involved in Fe-S Cluster Biosynthesis of Nitrogenase in WLY78.
Int J Mol Sci
April 2021
State Key Laboratory of Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, China.
NifS and NifU (encoded by and ) are generally dedicated to biogenesis of the nitrogenase Fe-S cluster in diazotrophs. However, and are not found in N-fixing strains, and the mechanisms involved in Fe-S cluster biosynthesis of nitrogenase is not clear. Here, we found that the genome of WLY78 contains the complete operon, a partial operon, a -like gene, two -like genes (-like and ), and two genes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!