Preparing Brain Slices to Study Basic Synaptic Properties.

Cold Spring Harb Protoc

Department of Biological Sciences, University at Buffalo, State University of New York, Buffalo, New York 14260.

Published: January 2017

AI Article Synopsis

  • This protocol outlines the steps for preparing brain slices used in electrophysiological studies, focusing on assessing synaptic physiology through voltage clamp techniques.
  • It emphasizes the importance of this method in exploring the characteristics of single neurons and their interconnections.
  • The technique is considered the gold standard in neuroscience for gaining insights into neuronal functions and synaptic interactions.

Article Abstract

This protocol describes how to prepare brain slices for electrophysiology, with an emphasis on synaptic physiology in voltage clamp. This approach remains the gold standard for understanding the properties of individual neurons, as well as the connections between neurons.

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Source
http://dx.doi.org/10.1101/pdb.prot093328DOI Listing

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