Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed by genes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen in to identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we term , which affects peroxisome abundance. encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants in , and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
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| http://dx.doi.org/10.1242/jcs.187914 | DOI Listing |
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