AI Article Synopsis
- Cytokinin Response Factors (CRFs) are transcription factors that play a key role in how plants respond to the plant hormone cytokinin.
- CRF proteins have a specific structure, including a dimerization domain, a DNA-binding domain, and a unique C-terminal region whose function isn't fully understood.
- Research on Arabidopsis CRF5 indicates that its C-terminal region can activate gene transcription, suggesting it has a modular structure that supports gene activation in a somewhat redundant manner.
Article Abstract
Cytokinin Response Factors (CRFs) are AP2/ERF transcription factors involved in cytokinin signal transduction. CRF proteins consist of a N-terminal dimerization domain (CRF domain), an AP2 DNA-binding domain, and a clade-specific C-terminal region of unknown function. Using a series of sequential deletions in yeast-2-hybrid assays, we provide evidence that the C-terminal region of Arabidopsis CRF5 can confer transactivation activity. Although comparative analyses identified evolutionarily conserved protein sequence within the C-terminal region, deletion experiments suggest that this transactivation domain has a partially redundant modular structure required for activation of target gene transcription.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351726 | PMC |
| http://dx.doi.org/10.1080/15592324.2016.1276684 | DOI Listing |
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