AI Article Synopsis
- Researchers developed a transgenic zebrafish model expressing luciferase to track hematopoietic cells' homing after being transplanted.
- Bioluminescence imaging (BLI) successfully monitored the movement of these cells to the bone marrow and the effects of compounds like prostaglandin E on their engraftment.
- A phytosterol, ergosterol, was identified that enhances hematopoietic cell homing and expands specific cell populations, showing the effectiveness of BLI in studying cellular behavior in real-time.
Article Abstract
To discover small molecules that modulate hematopoietic cell homing after adoptive transfer, we created a transgenic zebrafish expressing firefly luciferase downstream of the ubiquitin promoter (ubi:luc) to serve as a hematopoietic donor. Bioluminescence imaging (BLI) was used to detect and follow ubi:luc hematopoietic cells that homed to the marrow as early as 1 day post-transplant. BLI was able to detect the biological effect of prostaglandin E on early homing/engraftment of donor hematopoietic cells. This system was utilized in a functional screen of small molecules to enhance homing/engraftment. We discovered a phytosterol, ergosterol, that could increase hematopoietic cell homing in zebrafish and mice. In addition, ergosterol increased CXCR4 expression and promoted expansion of LinSCA-1KIT cells in vitro. We have demonstrated the utility of in vivo BLI to non-invasively monitor donor hematopoietic cell activity in adult zebrafish as a functional screen for mediators of cellular homing.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5233450 | PMC |
| http://dx.doi.org/10.1016/j.stemcr.2016.12.004 | DOI Listing |
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