Background: In this study, the effect of low concentrations of poly(amido)amine dendrimers (G2-G4) on human lymphocytes was studied. Some works revealed that PAMAMs can adversely affect the morphology of blood components and mitochondria functions. In this context, the present report aimed to investigate the in vitro cationic dendrimers' effect on mitochondrial respiration and cell morphology in lymphocytes isolated from human blood.
Methods: To monitor the mitochondrial changes, the high-resolution respirometer was used, whereas the cell morphology was analyzed using a flow cytometer and fluorescence microscopy.
Results: The concentration-dependent dendrimers' influence on lymphocytes morphology was shown. Changes in mitochondrial respiration revealed the concentration- and generation-dependent differences between dendrimer activity. There were no alterations in the routine respiration and in the state of the inner mitochondrial membrane (L/E), but decreased ADP- and FCCP-stimulated respirations were detected after treatment with G3 and G4 dendrimers. The markers of mitochondrial membrane integrity (RCR) and OXPHOS efficiency (P/E) significantly decreased regardless of the dendrimer generation used.
Conclusion: Based on these in vitro evaluations, we state that cationic PAMAM dendrimers can impair both the morphology and the bioenergetics of human lymphocytes, even when used at low concentrations and in a short time (up to 1 h). However, these results do not imply that similar findings could be possible for in vivo observations.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2174/1389201017666161229153018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Heterogeneity of Apolipoprotein B Levels Among Hispanic or Latino Individuals Residing in the US.
JAMA Cardiol
January 2025
Cardiology Division, Department of Medicine, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York.
Importance: Apolipoprotein B (apoB) distribution and its implications as an atherosclerotic cardiovascular disease (ASCVD) risk-enhancing factor among individuals of diverse Hispanic or Latino backgrounds have not been described.
Objective: To describe the distribution of apoB in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) cohort and to characterize associations of baseline sociodemographic and clinical variables with apoB and self-identified Hispanic or Latino background.
Design, Setting, And Participants: The HCHS/SOL was a prospective, population-based cohort study of diverse Hispanic or Latino adults living in the US who were recruited and screened between March 2008 and June 2011.
Facile preparation of a hydrophilic Eu-based ratiometric fluorescent nanosensor for Cu ion detection and imaging in living cells.
Anal Methods
January 2025
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430072, China.
In this work, a hydrophilic Eu-based ratiometric fluorescent nanosensor (PAAC-Eu) was developed for Cu ion detection in aqueous solutions and imaging in living cells. The sensor was prepared a simple one-step reaction at room temperature, leveraging the synergistic coordination of commercially accessible polyacrylic acid (PAA) and coumarin-3-carboxylic acid (CCAH) with Eu ions. PAAC-Eu was easy to disperse in aqueous media and exhibited two characteristic emission bands at 406 nm and 618 nm, respectively, upon excitation at 350 nm.
View Article and Find Full Text PDFSurface-enhanced Raman scattering of R6G dimerization during self-healing of gel.
Mikrochim Acta
January 2025
Centre for Micro and Nano Devices, Department of Physics, COMSATS Institute of Information Technology, Islamabad, 44500, Pakistan.
Traditional surface-enhanced Raman scattering (SERS) substrates seeking uniformity and reproducibility of the Raman signal often assume and require that hot spots remain consistently stable during Raman testing. Recently, the non-uniform accumulation in SERS sample pre-concentration strategies have inspired the direct use of self-healing noble metal aerogels (NMAs), as the sample pretreatment presented in this work, and uncovered more diverse Raman information of substances during the dynamic process of laser irradiation. Rare characteristic peaks such as 820 cm⁻ for R6G within a specific concentration range were observed, and potential processes including R6G dimerization and desorption were analyzed.
View Article and Find Full Text PDFDosing optimisation of intravenous lidocaine in patients with class 1-3 obesity by population pharmacokinetic analysis.
Anaesthesia
January 2025
Department of Anaesthesia and Perioperative Medicine, The Royal Brisbane and Women's Hospital, Brisbane, Australia.
Introduction: Evidence to support intra-operative lidocaine infusion regimens in patients with obesity is lacking, risking underdosing or toxicity. We aimed to measure the plasma concentrations of lidocaine and its active metabolites to develop a pharmacokinetic model and optimised dosing regimen in patients with obesity.
Methods: A standardised weight-based intravenous lidocaine regimen was administered to patients with a BMI ≥ 30 kg.
Deciphering the biosynthetic landscape of biofilms in glacier-fed streams.
mSystems
December 2024
River Ecosystems Laboratory, Alpine and Polar Environmental Research Center, Ecole Polytechnique Fédérale de Lausanne (EPFL), Sion, Switzerland.
Unlabelled: Glacier-fed streams are permanently cold, ultra-oligotrophic, and physically unstable environments, yet microbial life thrives in benthic biofilm communities. Within biofilms, microorganisms rely on secondary metabolites for communication and competition. However, the diversity and genetic potential of secondary metabolites in glacier-fed stream biofilms remain poorly understood.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!