Stringent control of FLP recombinase in Escherichia coli.

J Microbiol Methods

Biotechnology institute, 1500 Gortner Avenue, University of Minnesota, Saint Paul, MN 55108, United States; Department of Plant Biology, University of Minnesota, Saint Paul, MN 55108, United States. Electronic address:

Published: February 2017

Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.

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http://dx.doi.org/10.1016/j.mimet.2016.12.018DOI Listing

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