Assays for Identifying Inducers of the Antimicrobial Peptide LL-37.

Methods Mol Biol

Department of Laboratory Medicine, Karolinska Institutet, F68, Karolinska Universitetssjukhuset, Huddinge, 141 86, Sweden.

Published: February 2018

One promising approach to meet the growing problem of antibiotic resistance is to modulate host defense mechanisms, i.e., host-directed therapy (HDT), in the fight against infections. Induction of endogenous antimicrobial peptides (AMPs) via small molecular compounds, such as 1,25-dihydroxyvitamin D or phenylbutyrate, could provide one such HDT-based approach.We have developed a cell-based screening assay for the identification of novel compounds with the capacity to induce AMP expression and here follows the detailed protocol.

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http://dx.doi.org/10.1007/978-1-4939-6737-7_19DOI Listing

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