AI Article Synopsis
- The study focuses on how antimicrobial peptides interact with phospholipid membranes, which is important for understanding their function and effectiveness.
- Fluorescence correlation spectroscopy (FCS) is introduced as a valuable method to measure the leakage of fluorescent molecules from lipid vesicles, helping to identify the extent of membrane disruption caused by the peptides.
- The authors provide a comprehensive, step-by-step protocol for conducting an FCS-based leakage assay, along with practical tips to assist other researchers in replicating the work.
Article Abstract
There is considerable interest in understanding the interactions of antimicrobial peptides with phospholipid membranes. Fluorescence correlation spectroscopy (FCS) is a powerful experimental technique that can be used to gain insight into these interactions. Specifically, FCS can be used to quantify leakage of fluorescent molecules of different sizes from large unilamellar lipid vesicles, thereby providing a tool for estimating the size of peptide-induced membrane disruptions. If fluorescently labeled lipids are incorporated into the membranes of the vesicles, FCS can also be used to obtain information about whether leakage occurs due to localized membrane perturbations or global membrane destabilization. Here, we outline a detailed step-by-step protocol on how to optimally implement an FCS-based leakage assay. To make the protocol easily accessible to other researchers, it has been supplemented with a number of practical tips and tricks.
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| http://dx.doi.org/10.1007/978-1-4939-6737-7_11 | DOI Listing |
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