AI Article Synopsis
- Arachidonic acid (ARA) is a key polyunsaturated fatty acid in the brain, and its metabolic products have significant pharmacological effects.
- A challenge in studying ARA is the lack of commercially available uniformly labeled isotopes for research.
- This study presents a new protocol using Mortierella alpina to efficiently synthesize U-C-ARA from U-C-glucose, achieving high yields and purity for research applications.
Article Abstract
Arachidonic acid (ARA) is one of the most abundant polyunsaturated fatty acids (PUFAs) in the mammalian brain. Many enzymatically- and nonenzymatically-produced metabolic products have important and potent pharmacological properties. However, uniformly isotope labeled forms of ARA are not commercially available for studying the metabolic fates of ARA. This study describes a simple and efficient protocol for the biosynthesis of U-C-ARA from U-C-glucose, and U-C-ARA from U-C-glucose by Mortierella alpina. The protocols yield approximately 100nmol quantities of U-C-ARA with an isotopic purity of 95% from a 500μl batch volume, and approximately 2μCi quantities of U-C-ARA with an apparent specific activity in excess of 1200Ci/mol from a 250μl batch volume.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5237611 | PMC |
| http://dx.doi.org/10.1016/j.biortech.2016.12.050 | DOI Listing |
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