AI Article Synopsis
- Stevioside, a natural sweetener derived from Stevia leaves, is 300 times sweeter than sugar and can help lower blood sugar levels, benefiting diabetics.
- The study utilized tissue culture technology to produce disease-free stevia plants year-round and successfully identified stevioside content using High-Performance Liquid Chromatography (HPLC).
- The identified stevioside content in the leaf samples was 9.6%, suggesting a viable method for large-scale commercial production of stevia through in vitro culture globally.
Article Abstract
Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5167053 | PMC |
| http://dx.doi.org/10.4137/BCI.S30378 | DOI Listing |
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