Purpose: To investigate the effects of cAMP on transepithelial electrical parameters and fluid transport across porcine ciliary epithelium.
Methods: Transepithelial electrical parameters were determined by mounting freshly isolated porcine ciliary epithelium in a modified Ussing chamber. Similarly, fluid movement across intact ciliary body was measured with a custom-made fluid flow chamber.
Results: Addition of 1, 10, and 100 μM 8-Br-cAMP (cAMP) to the aqueous side (nonpigmented ciliary epithelium, NPE) induced a sustained increase in short-circuit current (Isc). Addition of niflumic acid (NFA) to the aqueous surface effectively blocked the cAMP-induced Isc stimulation. The administration of cAMP to the stromal side (pigmented ciliary epithelium, PE) triggered a significant stimulation of Isc only at 100 μM. No additive effect was observed with bilateral application of cAMP. Likewise, forskolin caused a significant stimulation of Isc when applied to the aqueous side. Concomitantly, cAMP and forskolin increased fluid transport across porcine ciliary epithelium, and this stimulation was effectively inhibited by aqueous NFA. Depleting Cl- in the bathing solution abolished the baseline Isc and inhibited the subsequent stimulation by cAMP. Pretreatment with protein kinase A (PKA) blockers (H89/KT5720) significantly inhibited the cAMP- and forskolin-induced Isc responses.
Conclusions: Our results suggest that cAMP triggers a sustained stimulation of Cl- and fluid transport across porcine ciliary epithelium; Cl- channels in the NPE cells are potentially a cellular site for this PKA-sensitive cAMP-mediated response.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1167/iovs.16-20127 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Kechuan Decoction mitigates apoptosis of airway epithelial cells by improving lipid metabolism disorders and mitochondria dysfunction in HDM-induced asthma.
Phytomedicine
November 2024
Jiangsu Key Laboratory of Children's Health and Chinese Medicine, State Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Changshu Hospital Affiliated to Nanjing University of Chinese Medicine, Changshu 215500, PR China. Electronic address:
Background: The airway epithelium serves as the first line of defense between the lung's internal environment and the external environment, functioning through physical barriers and mucus-ciliary clearance to protect against external allergens and other harmful substances. Airway epithelial damage is a common feature of asthma, and research has shown that apoptosis plays a significant role in airway injury and inflammation in asthma. Although Kechuan Decoction (KCD) has demonstrated clinical efficacy in treating pediatric asthma, its precise mechanism of action remains unclear.
View Article and Find Full Text PDFDirectional ciliary beats across epithelia require Ccdc57-mediated coupling between axonemal orientation and basal body polarity.
Nat Commun
November 2024
Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
Motile cilia unify their axonemal orientations (AOs), or beat directions, across epithelia to drive liquid flows. This planar polarity results from cytoskeleton-driven swiveling of basal foot (BF), a basal body (BB) appendage coincident with the AO, in response to regulatory cues. How and when the BF-AO relationship is established, however, are unaddressed.
View Article and Find Full Text PDFClinical features of midzonal iris pigment epithelial cysts: surprising findings revealed by ultrasound biomicroscopy.
Graefes Arch Clin Exp Ophthalmol
November 2024
Department of Ophthalmology, University of Cincinnati College of Medicine, Cincinnati, OH, 45267-0527, USA.
Article Synopsis
- The study examined midzonal iris pigment epithelial (IPE) cysts, which are usually harmless but can be confused with ciliary body melanoma, to better understand their characteristics using ultrasound biomicroscopy (UBM).
- Researchers reviewed 69 patient charts, finding that cysts were most common in older adults (median age 66), with a slight female majority and various cyst sizes, often located inferotemporally in the eye.
- UBM revealed that many cases thought to have multiple cysts were actually single cysts with distinct shapes, and associated peripheral IPE cysts were found in a majority of the evaluated eyes.
Uplift of genetic diagnosis of rare respiratory disease using airway epithelium transcriptome analysis.
Hum Mol Genet
November 2024
School of Human Development and Health, Institute for Developmental Sciences Building, Tremona Road, Southampton, Hampshire SO16 6YD, United Kingdom.
Rare genetic respiratory disease has an incidence rate of more than 1:2500 live births in Northern Europe and carries significant disease burden. Early diagnosis improves outcomes, but many individuals remain without a confident genetic diagnosis. Improved and expanded molecular testing methods are required to improve genetic diagnosis rates and thereby improve clinical outcomes.
View Article and Find Full Text PDFLife-long functional regeneration of in vivo airway epithelium by the engraftment of airway basal stem cells.
Nat Protoc
November 2024
Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, USA.
Article Synopsis
- * Detailed protocols are provided for preparing and expanding airway basal stem cells from mice and induced pluripotent stem cells, followed by methods for their transplantation into mice.
- * Successful engraftment leads to the restoration of key airway functions and cell types, with timelines for generating and using these stem cells outlined for researchers.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!