AI Article Synopsis
- The study investigates the molecular epidemiology of MERS-CoV in dromedaries from the UAE, presenting the first complete genome sequences from local animals.
- It identifies one novel strain in clade A and nine strains in clade B, revealing genetic diversity and potential recombination events among the viruses present in camels.
- Phylogenetic and molecular clock analyses suggest multiple origins of MERS-CoV in dromedaries, with evidence of distinct lineages circulating in both camels and humans.
Article Abstract
Little is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5180373 | PMC |
| http://dx.doi.org/10.1038/emi.2016.129 | DOI Listing |
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