Objectives/hypothesis: This preliminary study investigated whether auditory-perceptual judgments of voice quality by experienced speech language pathologists were associated with instructions given to speakers to manipulate specific laryngeal postures.
Study Design: Experimental, within-subject design.
Methods: Nine speakers were instructed and trained to manipulate three vocal parameters implicated in functional voice disorders-false vocal fold constriction, vocal fold mass, and larynx height-while reading a standard passage. Experienced judges rated these standard passages in terms of the widely used perceptual voice qualities breathiness, roughness, vocal strain, glottal fry, tone onset, tone color, loudness, and pitch.
Results: Between-subject factorial analysis of variance, controlling for judge unreliability, revealed some evidence that perceptual judgments are strongly associated with underlying laryngeal muscle activity that can then serve clinical planning of goals for intervention. Eta effect sizes were large for all dependent measures, ranging from 0.39 for pitch to 0.77 for strained.
Conclusions: Although these results are encouraging, they were obtained under ideal conditions. Further research is warranted.
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http://dx.doi.org/10.1016/j.jvoice.2016.10.008 | DOI Listing |
Acta Pharm Sin B
December 2024
Department of Translational Neuroscience, Jing'an District Centre Hospital of Shanghai, Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200032, China.
Spinal microglia and astrocytes are both involved in neuropathic and inflammatory pain, which may display sexual dimorphism. Here, we demonstrate that the sustained activation of spinal astrocytes and astrocyte-derived interleukin (IL)-17A promotes the progression of mouse bone cancer pain without sex differences. Chemogenetic or pharmacological inhibition of spinal astrocytes effectively ameliorates bone cancer-induced pain-like behaviors.
View Article and Find Full Text PDFJ Tradit Complement Med
January 2025
Chinese Medicine Research Center, China Medical University, Taichung, Taiwan.
The sensation of sng (pronounced/səŋ/, the Romanization form of or soreness in Taiwanese Southern Min) associated with a composite of unique sensations, is a novel phenotype for acupoint stimulation. It is perceived by test participants but also by experienced practitioners as a sensation of "taking the bait" (by fish when fishing), a characteristic heavy and tight sensation from the needle. Here, we propose that sng is a powerful biomarker for associated with successful manual acupuncture.
View Article and Find Full Text PDFNat Plants
January 2025
Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China.
Precise manipulation of genome structural variations holds great potential for plant trait improvement and biological research. Here we present a genome-editing approach, dual prime editing (DualPE), that efficiently facilitates precise deletion, replacement and inversion of large DNA fragments in plants. In our experiments, DualPE enabled the production of specific genomic deletions ranging from ~500 bp to 2 Mb in wheat protoplasts and plants.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
January 2025
Nanjing University, School of Chemistry and Chemical Engineering, CHINA.
Proximity labeling (PL) has emerged as a powerful technique for the in situ elucidation of biomolecular interaction networks. However, PL methods generally rely on single-biological-hierarchy control of spatial localization at the labeling site, which limits their application in multi-tiered biological systems. Here, we introduced another enzymatic reaction upstream of an enzyme-based PL reaction and targeted the two enzymes to markers indicating different biological hierarchies, establishing a two-level spatially localized proximity labeling (P2L) platform for in situ molecular measurement and manipulation.
View Article and Find Full Text PDFExp Anim
January 2025
Research Institute for Microbial Diseases, Osaka University.
In mammals, blastocyst-stage trophectoderm (TE) contacts the maternal body at the time of implantation and forms the placenta after implantation, which supports the development of the fetus. Studying gene function in TE and placenta is important to understand normal implantation and pregnancy processes and their dysfunction. However, genetically modified mice are commonly generated by manipulating pronuclear-stage zygotes, which modify both the genome of the fetus and the placenta.
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