AI Article Synopsis
Article Abstract
Purpose: To assess the uptake, accumulation, temporal stability, and spatial localization of isoflurane (ISO) in the C57BL/6 mouse, and to identify its potential interference with the detection of labeled cardiac progenitor cells using F MRI/MR spectroscopy (MRS).
Materials And Methods: Objectives are demonstrated using (a) in vitro ISO tests, (b) in vivo temporal accumulation/spatial localization C57BL/6 studies (n = 3), and (c) through injections of perfluoro-crown-ether (PFCE) labeled cardiac progenitor cells into femoral muscle areas of the murine hindlimb post-mortem (n = 1) using H/ F MRI/MRS at 9.4 Tesla. Data were acquired using double-gated spoiled gradient echo images and pulse-acquire spectra. For the in vivo study, the temporal stability of ISO resonances was quantified using coefficient of variability (CV) (5 min) estimates.
Results: Two ISO resonances were observed in vivo that correspond to the -CF and -OCHF moieties. CV values ranged between 3.2 and 6.4% (-CF ) and 6.4 and 11.2% (-OCHF ). Reductions of the ISO dose (2.0 to 1.7%) at 80 min postinduction had insignificant effects on ISO signals (P = 0.23; P = 0.71). PFCE-labeled cells exhibited a resonance at -16.25 ppm in vitro that did not overlap with the ISO resonances, a finding that is confirmed with MRS post-mortem using injected, labeled cells. Based on F MRI, similar in vivo/post-mortem ISO compartmentalization was also confirmed in peripheral and thoracic skeletal muscles.
Conclusion: Significant ISO accumulation was observed by F MRS in vivo with temporally stable signals over 90 min postinduction. ISO effects on PFCE labels are anticipated to be minimal but may be more prominent for perfluoropolyether or perfluorooctyl bromide labels.
Level Of Evidence: 1 Technical Efficacy: Stage 1 J. MAGN. RESON. IMAGING 2017;45:1659-1667.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484368 | PMC |
| http://dx.doi.org/10.1002/jmri.25564 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synthetic organizer cells guide development via spatial and biochemical instructions.
Cell
December 2024
Cell Design Institute and Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address:
In vitro development relies primarily on treating progenitor cells with media-borne morphogens and thus lacks native-like spatial information. Here, we engineer morphogen-secreting organizer cells programmed to self-assemble, via cell adhesion, around mouse embryonic stem (ES) cells in defined architectures. By inducing the morphogen WNT3A and its antagonist DKK1 from organizer cells, we generated diverse morphogen gradients, varying in range and steepness.
View Article and Find Full Text PDFHeterogeneous Cardiac-Derived and Neural Crest-Derived Aortic Smooth Muscle Cells Exhibit Similar Transcriptional Changes After TGFβ Signaling Disruption.
Arterioscler Thromb Vasc Biol
December 2024
Department of Surgery (Cardiac), Yale School of Medicine, New Haven, CT. (P.R., B.J., A.H., G.L., W.L., R.A., G.T.).
Background: Smooth muscle cells (SMCs) of cardiac and neural crest origin contribute to the developing proximal aorta and are linked to disease propensity in adults.
Methods: We analyzed single-cell transcriptomes of aortic SMCs from adult mice to determine basal states and changes after disrupting TGFβ (transforming growth factor-β) signaling necessary for aortic homeostasis.
Results: A minority of Myh11 lineage-marked SMCs differentially expressed genes suggestive of embryological origin.
Comparative in situ characterization of erythroid cells found throughout the developing tongue tissue, circulation, and liver of fetal mice.
Ann Anat
December 2024
Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, Tokyo, Japan. Electronic address:
Background: Erythroid cells contribute to embryonic organ development and adult tissue repair supplying oxygen to tissues. During mouse development, the primitive erythroid cells produced in the extraembryonic blood islands of the yolk sac begin to circulate as immature and nucleated erythroblasts with the onset of cardiac contractions around embryonic day 9.5 (E9.
View Article and Find Full Text PDFExploring novel markers for coronary heart disease associated with systemic lupus erythematosus: A review.
Medicine (Baltimore)
December 2024
Affiliated Hospital of Shandong Second Medical University, School of Clinical Medicine, Weifang, China.
Systemic lupus erythematosus (SLE) is an autoimmune condition that is characterized by the production of autoantibodies and sustained inflammatory damage. Coronary heart disease (CHD) is a common complication of SLE, significantly increases CHD-related mortality in SLE patients. Despite conventional risk factors, the mechanisms contributing to a higher CHD risk require further investigation, with the immune and inflammatory aspects of SLE playing a significant role.
View Article and Find Full Text PDFGenome-Wide Expression Profiling and Phenotypic Analysis of Downstream Targets Identify the Fox Transcription Factor Jumeau as a Master Regulator of Cardiac Progenitor Cell Division.
Int J Mol Sci
December 2024
Department of Biology, Indiana State University, Terre Haute, IN 47809, USA.
Article Synopsis
- - Forkhead box (Fox) transcription factors (TFs) are crucial for heart development in mammals, influencing cardiac progenitor cell roles and positions.
- - Researchers found that the Jumu gene plays a key regulatory role, with many downstream targets linked to cell division in cardiac progenitor cells.
- - The study highlights Jumu as a central hub in a network that controls cardiac progenitor cell division, supported by the interactions of identified gene targets.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!