AI Article Synopsis
- The automatic optical fractionator is a new stereology method that allows for unbiased and efficient counting of cells in tissue sections.
- It works with existing image processing techniques and immunostaining to produce automated cell counts from 3D tissue images, making it significantly faster and more accurate than manual methods.
- The study highlights the importance of using a high-resolution lens to achieve a thin focal plane, which is critical for accurate cell counting with this technique.
Article Abstract
A novel stereology approach, the automatic optical fractionator, is presented for obtaining unbiased and efficient estimates of the number of cells in tissue sections. Used in combination with existing segmentation algorithms and ordinary immunostaining methods, automatic estimates of cell number are obtainable from extended depth of field images built from three-dimensional volumes of tissue (disector stacks). The automatic optical fractionator is more accurate, 100% objective and 8-10 times faster than the manual optical fractionator. An example of the automatic fractionator is provided for counts of immunostained neurons in neocortex of a genetically modified mouse model of neurodegeneration. Evidence is presented for the often overlooked prerequisite that accurate counting by the optical fractionator requires a thin focal plane generated by a high optical resolution lens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5303677 | PMC |
| http://dx.doi.org/10.1016/j.jchemneu.2016.12.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Microfluidic fractionation of microplastics, bacteria and microalgae with induced-charge electro-osmotic eddies.
Anal Chim Acta
February 2025
School of Control Engineering, Northeastern University at Qinhuangdao, Qinhuangdao, 066004, PR China; Hebei Key Laboratory of Micro-Nano Precision Optical Sensing and Measurement Technology, Qinhuangdao, 066004, PR China. Electronic address:
Background: Fractionation of microalgal cells has important applications in producing pharmaceuticals and treating diseases. Multiple types of microalgal cells generally coexist in the oceans or lakes and are easily contaminated by microplastics and bacteria. Therefore, it is of paramount significance to develop an effective fractionation approach for microalgal cells for biological applications.
View Article and Find Full Text PDFQuality Investigation of Pultruded Carbon Fiber Panels Subjected to Four-Point Flexure via Fiber Optic Sensing.
Materials (Basel)
January 2025
Tickle College of Engineering, University of Tennessee, Knoxville, TN 37996, USA.
Pultruded carbon fiber-reinforced composites are attractive to the wind energy industry due to the rapid production of highly aligned unidirectional composites with enhanced fiber volume fractions and increased specific strength and stiffness. However, high volume carbon fiber manufacturing remains cost-prohibitive. This study investigates the feasibility of a pultruded low-cost textile carbon fiber-reinforced epoxy composite as a promising material in spar cap production was undertaken based on mechanical response to four-point flexure loading.
View Article and Find Full Text PDFFractionation and identification of ocular protective compounds from kochiae fructus against oxidative damage in retinal pigment epithelium cells.
J Ethnopharmacol
January 2025
Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung, 40447, Taiwan; Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Master Program of Pharmaceutical Manufacture, College of Pharmacy, China Medical University, Taichung, 40402, Taiwan; Department of Medical Laboratory Science and Biotechnology, College of Medical and Health Science, Asia University, Taichung, 41354, Taiwan. Electronic address:
Ethnopharmacological Relevance: Kochiae Fructus, the ripe fruit of Kochia scoparia, is a traditional Chinese medicine commonly used to treat eye discomforts and vision problems. Although Kochiae Fructus is mentioned in many classical Chinese medical texts, its protective effects and the roles of its active phytochemicals in eye treatment still lack scientific exploration.
Aim Of The Study: This study aimed to clarify the protective effects and identify the active fractions and compounds of Kochiae Fructus against oxidative stress-induced retinal pigment epithelium (RPE) cell death.
Particulate matter and potentially toxic element content in urban ornamental plant species to assess pollutants trapping capacity.
J Environ Manage
January 2025
Department of Plant Biology and Ecology, University of Seville, Avda. Reina Mercedes S/n, Apartado de Correos, 1095, 41012, Sevilla, Spain. Electronic address:
Urban environments are usually polluted by anthropogenic activities like traffic, a major source of potentially toxic elements (PTEs), and ornamental plant species may reduce contamination by trapping traffic-related air pollutants in their leaves. The purpose of this study was tested the trapping pollutant capacity of four species commonly used in green areas of Seville city (SW Spain) to better choose species in urban green planning. Composition of particulate matter (PM) obtained from foliar surfaces (sPM) and wax-included (wPM) was determined by EDX-SEM analysis in samples from different city locations.
View Article and Find Full Text PDFIsolation of anti-inflammatory and cytotoxic secondary metabolites from Valeriana phu and evaluation of their mechanisms of action.
Fitoterapia
January 2025
Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR-34755, Kayışdağı, İstanbul, Türkiye. Electronic address:
As a result of anti-inflammatory activity-guided fractionation, 16 secondary metabolites from the underground parts of Valeriana phu L. were obtained, including five new ones belonging to iridoid (1, 2, and 5), phenylpropanoid (6) and neolignan (7) chemical classes. Their structures were elucidated by 1D and 2D NMR analyses as well as HRESIMS.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!