One-pot synthesis of injectable methylcellulose hydrogel containing calcium phosphate nanoparticles.

A thermo-sensitive hydrogel based on calcium phosphate nanoparticles was prepared using a novel one-pot method in which added precursor salts induced a salt-out effect in the methylcellulose (MC) solution. The calcium phosphate nanoparticles were synthesized in situ from precursor ions (Ca and HPO) in the hydrogel, and residual ions (HPO and Cl) in the formation of the nanoparticles decreased the gelation temperature of the MC solution. This one-pot synthesis was an effective way to streamline the previous multi-step process and to easily prepare a thermo-sensitive hydrogel containing bioactive nanoparticles. The thermo-sensitive properties of different concentrations of MC hydrogels were characterized by a rheometer and infrared (IR) spectroscopy. The gelation behavior of MC according to the addition of various salts was monitored by rheometer. With respect to a selected concentration and precursor, the structure of the MC hydrogel containing calcium phosphate nanoparticles was analyzed by scanning electron microscopy (SEM), X-ray diffraction (XRD) and IR.