AI Article Synopsis
- MicroRNAs (miRNAs) are important regulatory elements in mammals that interact with transcribed ultraconserved regions (T-UCRs), affecting gene expression processes.
- Evidence shows that manipulating one component of the miRNA-T-UCR network in bladder cancer cells can influence the expression of other components, indicating a tightly interconnected system.
- The interaction between miRNAs, T-UCRs, and the polycomb protein YY1 reveals a new layer of gene expression regulation that has implications for understanding cancer biology.
Article Abstract
MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192503 | PMC |
| http://dx.doi.org/10.3390/genes7120127 | DOI Listing |
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