Fusarielins are polyketides with a decalin core produced by various species of and . Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in a strain overexpressing the local transcription factor. The results suggest that a -acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 in biosynthesis of a polyketide product, which is released by hydrolysis by a -acting thioesterase (FSL2). Deletion of the epimerase (FSL3) resulted in accumulation of an unstable compound, which could be the released product. A novel compound, named prefusarielin, accumulated in the deletion mutant of the cytochrome P450 monooxygenase FSL4. Unlike the known fusarielins from , this compound does not contain oxygenized decalin rings, suggesting that FSL4 is responsible for the oxygenation.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274466 | PMC |
| http://dx.doi.org/10.3390/molecules21121710 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genomic investigation of plant secondary metabolism: insights from synteny network analysis of oxidosqualene cyclase flanking genes.
New Phytol
December 2024
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100, China.
The clustered distribution of genes involved in metabolic pathways within the plant genome has garnered significant attention from researchers. By comparing and analyzing changes in the flanking regions of metabolic genes across a diverse array of species, we can enhance our understanding of the formation and distribution of biosynthetic gene clusters (BGCs). In this study, we have designed a workflow that uncovers and assesses conserved positional relationships between genes in various species by using synteny neighborhood networks (SNN).
View Article and Find Full Text PDFLoss of Hoxa5 function affects Hox gene expression in different biological contexts.
Sci Rep
December 2024
Centre de Recherche sur le Cancer de L'Université Laval, Centre de Recherche du CHU de Québec-Université Laval (Oncology), 1401, 18e Rue, Québec, QC, G1J 1Z4, Canada.
Hoxa5 plays numerous roles in development, but its downstream molecular effects are mostly unknown. We applied bulk RNA-seq assays to characterize the transcriptional impact of the loss of Hoxa5 gene function in seven different biological contexts, including developing respiratory and musculoskeletal tissues that present phenotypes in Hoxa5 mouse mutants. This global analysis revealed few common transcriptional changes, suggesting that HOXA5 acts mainly via the regulation of context-specific effectors.
View Article and Find Full Text PDFDiagnostic value and immune infiltration characterization of WTAP as a critical m6A regulator in liver transplantation.
Hepatobiliary Pancreat Dis Int
December 2024
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400000, China. Electronic address:
Background: RNA N6-methyladenosine (m6A) regulators are essential for numerous biological processes and are implicated in various diseases. However, the comprehensive role of m6A regulators in the context of liver transplantation (LT) remains poorly understood. This study aimed to illustrate the relationship between m6A regulators and ischemia-reperfusion injury (IRI) following LT.
View Article and Find Full Text PDFCognitive decline and neuroinflammation in a mouse model of obesity: An accelerating role of ageing.
Brain Behav Immun
December 2024
Department of Geriatrics, University Medical Center Goettingen, Robert-Koch-Str. 42, 37075 Goettingen, Germany; DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, 37075 Göttingen, Germany. Electronic address:
Obesity, a pandemic, worldwide afflicts almost one billion people. Obesity and ageing share several pathological pathways leading to neurological disorders. However, due to a lack of suitable animal models, the long-term effects of obesity on age-related disorders- cognitive impairment and dementia have not yet been thoroughly investigated.
View Article and Find Full Text PDFContributions to the () Group in China: Taxonomy, Species Diversity, and Molecular Phylogeny.
J Fungi (Basel)
December 2024
Engineering Research Center of Tropical Medicine Innovation and Transformation of Ministry of Education, International Joint Research Center of Human-Machine Intelligent Collaborative for Tumor Precision Diagnosis and Treatment of Hainan Province, Hainan Provincial Key Laboratory of Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China.
is the largest genus in the family , with approximately 1000 species worldwide. Basic data on the species diversity, geographic distribution, and the infrageneric framework of are still incomplete because of the intricate nature of this genus, which includes numerous unrecognized taxa that exist around the world. A multigene phylogeny of the group, initially designated as the " subgroup", was conducted using the ITS-28S- nucleotide datasets.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!