AI Article Synopsis
- A new type of magnetoliposomes (MLs) was developed for detecting coenzyme Q10 (CoQ10), using hydrophobic magnetic-gold nanoparticles and a fluorophore called cresyl violet.
- The MLs are concentrated prior to detection through a flow system and an electromagnet, and their lysis through Triton X-100 leads to a decrease in fluorescence that indicates CoQ10 levels.
- The method allows for accurate measurements in food samples, with a calibration range of 0.03-0.50 μmol/L, a detection limit of 0.008 μmol/L, and recovery rates between 83.5-101.3% when compared to traditional chromatographic techniques.
Article Abstract
A new type of magnetoliposomes (MLs), containing hydrophobic magnetic-gold nanoparticles and the long wavelength fluorophore cresyl violet, has been used for the determination of coenzyme Q10 (CoQ10). MLs were concentrated just before the detector, using a flow system and an external electromagnet device. The subsequent introduction of Triton X-100 and CoQ10 causes the MLs lysis and the cresyl violet oxidation, obtaining a decrease in the fluorescence signal. The dynamic range of the calibration graph was 0.03-0.50μmolL CoQ10, and the detection limit was 0.008μmolL. The precision (relative standard deviation) was in the range of 1.3-4.5%. The method showed a sampling frequency of 12h and was applied to the determination of CoQ10 in several food samples. The results were compared with those obtained using a previously described chromatographic method. Also, recovery values were in the range of 83.5-101.3%.
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| http://dx.doi.org/10.1016/j.foodchem.2016.11.085 | DOI Listing |
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