Hydrogen selenide (HSe) is an important metabolite of dietary Se compounds and has been implicated in various pathological and physiological processes. The development of highly sensitive and selective methods for the sensing of HSe is therefore very important. Herein, we developed a fluorescent probe (hemicyanine (Hcy)-HSe) for detecting HSe based on a new HSe-specific receptor unit, 1,2-dithiane-4,5-diol. Hcy-HSe showed high selectivity toward HSe over thiols (RSH), hydrogen sulfide (HS), and selenocysteine (Sec) and was further exploited for the fluorescence imaging of HSe both in living cells and in vivo. Furthermore, with the aid of Hcy-HSe, we demonstrated that HSe can be generated and gradually accumulated in HepG2 cells under hypoxic conditions and in the solid tumor after treatment with NaSeO.
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