AI Article Synopsis
- - Hydrogenases, ferredoxins, and FNR (ferredoxin-NADP reductases) are crucial redox proteins involved in electron metabolism and have potential applications in creating biological hydrogen production technologies.
- - A novel high-throughput H production assay device (H PAD) allows for the simultaneous testing of 96 reactions, utilizing image analysis of thin films to monitor hydrogen production efficacy.
- - The H PAD helped discover improved mutants of hydrogenase and FNR, achieving nearly 3-fold and 60% increases in hydrogen production rates respectively, while also aiding in understanding biochemical processes.
Article Abstract
Hydrogenases, ferredoxins, and ferredoxin-NADP reductases (FNR) are redox proteins that mediate electron metabolism in vivo, and are also potential components for biological H production technologies. A high-throughput H production assay device (H PAD) is presented that enables simultaneous evaluation of 96 individual H production reactions to identify components that improve performance. Using a CCD camera and image analysis software, H PAD senses the chemo-optical response of Pd/WO thin films to the H produced. H PAD-enabled discovery of hydrogenase and FNR mutants that enhance biological H production is reported. From a library of 10 080 randomly mutated Clostridium pasteurianum [FeFe] hydrogenases, we found a mutant with nearly 3-fold higher H production specific activity. From a library of 400 semi-randomly mutated Oryza sativa FNR, the top hit enabled a 60 % increase in NADPH-driven H production rates. H PAD can also facilitate elucidation of fundamental biochemical mechanisms within these systems.
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| http://dx.doi.org/10.1002/anie.201610260 | DOI Listing |
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