This paper is devoted to the development of a steady-state behavior of a quantum dot-semiconductor optical amplifier (QD-SOA). The investigated performance characteristics cover a wide range that includes material gain coefficient, spatial distribution of the occupation probabilities, fiber to fiber gain, gain spectrum as a function of the bias current, relaxation time, and capture time. A set of traveling-wave equations is used to model the signal and spontaneous photons along the device active region. The obtained results indicate a high gain that reaches 34 dB for an InAs/InGaAsP/InP-based QD-SOA, with a corresponding device length of 4 mm. The obtained signal-to-noise ratio is larger than 75 dB for all input powers without using an output filter.
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http://dx.doi.org/10.1364/AO.55.009978 | DOI Listing |
Genes (Basel)
December 2024
The School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, D02 VF25 Dublin, Ireland.
Background: An estimated 10-15% of all genetic diseases are attributable to variants in noncanonical splice sites, auxiliary splice sites and deep-intronic variants. Most of these unstudied variants are classified as variants of uncertain significance (VUS), which are not clinically actionable. This study investigated two novel splice-altering variants, NM_000390.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
January 2025
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry and Materials Sciences, Hebei University, Baoding 071002, Hebei, China.
Screening carbonyl reductases with the ability to catalyze the reduction of complex carbonyl compounds is of great significance for the biosynthesis of -tolvaptan(-TVP). In this study, the target carbonyl reductase in the crude enzyme extract of rabbit liver was separated, purified, and identified by ammonium sulfate precipitation, gel-filtration chromatography, ion exchange chromatography, affinity chromatography, and protein mass spectrometry. With the rabbit liver genome as the template, the gene encoding the carbonyl reductase was amplified by PCR and the recombinant strain was successfully constructed.
View Article and Find Full Text PDFBiosens Bioelectron
January 2025
Education Department of Guangxi Zhuang Autonomous Region, Laboratory of Optic-electric Chemo/Biosensing and Molecular Recognition, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, School of Chemistry and Chemical Engineering, Guangxi Minzu University, Nanning 530006, China. Electronic address:
Sugarcane smut is a widespread fungal disease, which severely impairs the quality and sugar yield of sugarcane. Early detection is crucial for mitigating its impact, which makes the development of a highly sensitive and accurate detection method essential. Herein, the Mn-doped zeolite imidazolate framework (ZIF-67), synthesized via a nano-confined-reactor approach, is designed to significantly enhance electron transport and boost the enzyme loading capacity within biofuel cells, thereby potentially enhancing their overall performance.
View Article and Find Full Text PDFNat Commun
January 2025
NMR-based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany.
Membrane bound histidine kinases (HKs) are ubiquitous sensors of extracellular stimuli in bacteria. However, a uniform structural model is still missing for their transmembrane signaling mechanism. Here, we used solid-state NMR in conjunction with crystallography, solution NMR and distance measurements to investigate the transmembrane signaling mechanism of a paradigmatic citrate sensing membrane embedded HK, CitA.
View Article and Find Full Text PDFCureus
December 2024
Department of Clinical Research, University of San Luis Potosí, San Luis Potosí, MEX.
Background: In large-scale molecular studies, a protocol that generates high yields and quality DNA for future polymerase chain reaction (PCR) assays is needed. The collection of buccal cells by cytobrush may represent an efficient, noninvasive, and inexpensive method for obtaining genetic material from school populations. The aim of this study was to develop a method to obtain genomic DNA from buccal cells of schoolchildren, and the DNA was extracted immediately after collecting the buccal cell samples and after storing the samples for 8 months at -20 °C to establish the feasibility of the method for epidemiological studies.
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