AI Article Synopsis
- Uric acid (UA) has neuroprotective properties, but the way it is regulated in the brain is not fully understood, prompting further investigation into UA transporters in murine brains.* -
- The study employed immunostaining and in situ hybridization to identify the distribution of several UA transporters, including GLUT9, ABCG2, and URAT1 in different brain cell types.* -
- Findings suggest that specific UA transporters are expressed in ependymal cells, choroid plexus epithelium, and brain capillaries, indicating potential pathways for targeted neuroprotection using these transporters.*
Article Abstract
Background: Uric acid (UA) is known to exert neuroprotective effects in the brain. However, the mechanism of UA regulation in the brain is not well characterized. In our previous study, we described that the mouse urate transporter URAT1 is localized to the cilia and apical surface of ventricular ependymal cells. To further strengthen the hypothesis that UA is transported transcellularly at the ependymal cells, we aimed to assess the distribution of other UA transporters in the murine brain.
Methods: Immunostaining and highly-sensitive in situ hybridization was used to assess the distribution of UA transporters: GLUT9/URATv1, ABCG2, and URAT1.
Results: Immunostaining for GLUT9 was observed in ependymal cells, neurons, and brain capillaries. Immunostaining for ABCG2 was observed in the choroid plexus epithelium and brain capillaries, but not in ependymal cells. These results were validated by in situ hybridization.
Conclusions: We propose that given their specific expression patterns in ependymal, choroid plexus epithelial, and brain capillary endothelial cells in this study, UA may be transported by these UA transporters in the murine brain. This may provide a novel strategy for targeted neuroprotection.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5154092 | PMC |
| http://dx.doi.org/10.1186/s12987-016-0046-x | DOI Listing |
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