A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 μg/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (<5%), and the results were easy to interpret without any sophisticated equipment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.6b04157 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Quantitative determination of leptin hormone using gold nanoparticle-based lateral flow assay.
Mikrochim Acta
January 2025
Science and Technology Research and Application Center (BITAM), Necmettin Erbakan University, Konya, Türkiye.
A lateral flow assay (LFA) has been developed that can be used in point-of-care (PoC) use for the sensitive determination of leptin hormone. The limit of detection value was 0.158 ng/mL and the limit of quantification value was 0.
View Article and Find Full Text PDFPortable paper-based microfluidic devices with CuAgS NPs modification for multiplex intelligent visualized detection of adrenaline and glucose simultaneously.
Anal Chim Acta
January 2025
Center for Quantum Sciences and School of Physics, Northeast Normal University, Changchun, 130117, China.
Background: Adrenaline and glucose are essential biomarkers in human body for maintaining metabolic balance. Abnormal levels of adrenaline and glucose are associated with various diseases. Therefore, it is important to design portable, on-site devices for rapid adrenaline and glucose analysis to safeguard health.
View Article and Find Full Text PDFPreparation of peptides against immunocomplex of deltamethrin and application in noncompetitive lateral flow immunoassay.
Food Chem
January 2025
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China. Electronic address:
Lateral flow immunoassay (LFIA) has the advantages of simplicity and rapidness, and is widely used for the rapid detection of pesticides and other analytes. However, small molecule compounds such as pesticides are often analyzed using competitive LFIA (CLFIA), whose sensitivity often does not meet the actual needs. In this study, a noncompetitive LFIA (NLFIA) for deltamethrin (DM) with high sensitivity was developed by using anti-immunocomplex peptides (AIcPs).
View Article and Find Full Text PDFCoupling tree-ring and geomorphic analyses to reconstruct the 1950s massive Glacier Lake Outburst Flood at Grosse Glacier, Chilean Patagonia.
Sci Total Environ
January 2025
Climate Change Impacts and Risks in the Anthropocene (C-CIA), Institute for Environmental Sciences, University of Geneva, Geneva, Switzerland; dendrolab.ch, Department of Earth Sciences, University of Geneva, Geneva, Switzerland; Department F.-A. Forel for Environmental and Aquatic Sciences, University of Geneva, Switzerland.
Over recent decades, global warming has led to sustained glacier mass reduction and the formation of glacier lakes dammed by potentially unstable moraines. When such dams break, devastating Glacial Lake Outburst Floods (GLOFs) can occur in high mountain environments with catastrophic effects on populations and infrastructure. To understand the occurrence of GLOFs in space and time, build frequency-magnitude relationships for disaster risk reduction or identify regional links between GLOF frequency and climate warming, comprehensive databases are critically needed.
View Article and Find Full Text PDFUltrasensitive "Hunter of Target": Rabbit Monoclonal Antibody-Based Competitive Lateral Flow Immunoassay for Saxitoxin.
Anal Chem
January 2025
Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
The rapid, sensitive, and accurate detection of paralytic shellfish toxins (PSTs), such as saxitoxin (STX), is critical for protecting human health due to the frequent occurrence of toxic red tides. In this work, to address the low affinity of traditional mouse monoclonal antibodies (m-mAbs), rabbit monoclonal antibodies (r-mAbs) against STX were produced by a single B-cell sorting culture and a cross-selection strategy. The r-mAbs showed 100-fold improvement in sensitivity (IC = 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!