Ginsenoside Rg1 is a valuable bioactive molecule but its high polarity and low concentration in complex mixtures makes it a challenge to separate Ginsenoside Rg1 from other saponins with similar structures, resulting in low extraction efficiency. The successful development of effective Rg1 molecularly imprinted polymers that exhibit high selectivity and adsorption may offer an improved method for the enrichment of active compounds. In this work, molecularly imprinted polymers were prepared with two different methods, precipitation polymerization or surface imprinted polymerization. Comparison of the adsorption abilities showed higher adsorption of the surface molecularly imprinted polymers prepared by surface imprinted polymerization, 46.80 mg/g, compared to the 27.74 mg/g observed for the molecularly imprinted polymers prepared by precipitation polymerization. Therefore, for higher adsorption of the highly polar Rg1, surface imprinted polymerization is a superior technique to make Rg1 molecularly imprinted polymers. The prepared surface molecularly imprinted polymers were tested as a solid-phase extraction column to directionally enrich Rg1 and its analogues from ginseng tea and total ginseng extracts. The column with surface molecularly imprinted polymers showed higher enrichment efficiency and better selectivity than a C solid-phase extraction column. Overall, a new, innovative method was developed to efficiently enrich high-polarity bioactive molecules present at low concentrations in complex matrices.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jssc.201601193 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Rethinking the use of redox probes for the detection of electroactive proteins with electrochemical sensors modified with molecularly imprinted polymers.
Biosens Bioelectron
December 2024
Cnam, SATIE Laboratory, UMR, CNRS 8029, 292 rue Saint Martin, 75003, Paris, France. Electronic address:
This study aims to demonstrate that redox couples, regardless of their electrical charges, are unnecessary for detecting and quantifying electroactive proteins using an electrochemical sensor functionalized with a molecularly imprinted polymer. Our approach involved designing a polydopamine imprinted biosensor for detecting bovine serum albumin as the model protein. Electrochemical measurements were conducted in a phosphate-buffered solution (PBS) and solutions containing the negatively charged hexacyanoferrate, the neutral ferrocene, or the positively charged hexaammineruthenium (III) probes.
View Article and Find Full Text PDFAn antibody-free bio-layer interferometry biosensor for immunoglobulin G1 detection in human serum by using molecularly imprinted polynorepinephrine.
Biosens Bioelectron
December 2024
Department of Chemistry "Ugo Schiff', University of Florence, Via della Lastruccia, 3-13, 50019, Sesto Fiorentino, Italy. Electronic address:
Bio-Layer Interferometry (BLI) has emerged as a versatile technique in affinity-based biosensing, analogous to Surface Plasmon Resonance. BLI enables real-time, label-free detection, and quantification of biomolecular interactions between an immobilized receptor and an analyte in solution. The BLI sensor comprises an optical fiber with an internal reference layer at the end and an external biocompatible layer where biological receptors are immobilized and exposed to the solution.
View Article and Find Full Text PDFA Review Study on Molecularly Imprinting Surface Plasmon Resonance Sensors for Food Analysis.
Biosensors (Basel)
November 2024
Department of Nutrition and Dietetics, Faculty of Health Sciences, Hasan Kalyoncu University, Gaziantep 27000, Turkey.
Surface plasmon resonance (SPR) sensors have emerged as a powerful tool in biosensing applications due to their ability to provide sensitive and real-time detection of chemical and biological analytes. This review focuses on the development and application of molecularly imprinted polymer (MIP)-based SPR sensors for food analysis. By combining the high selectivity of molecular imprinting techniques with the sensitivity of SPR, these sensors offer significant advantages in detecting food contaminants and other target molecules.
View Article and Find Full Text PDFFabrication of bio-mimic nanozyme based on Mxene@AuNPs and molecular imprinted poly(thionine) films for creatinine detection.
Biosens Bioelectron
December 2024
Department of Cogno-Mechatronics Engineering, College of Nanoscience and Nanotechnology, Pusan National University, Busan, 46241, Republic of Korea. Electronic address:
Creatinine (Ctn) is a biomarker for chronic kidney disease (CKD). In this study, a highly sensitive and specific detection method for Ctn based on a molecularly imprinted polymer (MIP) based electrochemical biosensor was developed. Mxene (Mx), which has high absorption properties, was modified using carbon screen-printed electrodes (SPCE).
View Article and Find Full Text PDFA dual-functional monomer-based molecularly imprinted fluorescent aptasensor employing near-infrared carbon dots for selective detection of quinine in food.
Food Chem
December 2024
Department of Chemistry, College of Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address:
A molecularly imprinted fluorescent aptasensor was designed for selective detection of quinine (Qn) based on dual functional monomers. In the sol-gel polymerization of molecularly imprinted polymers (MIPs), 3-aminopropyltriethoxysilane (APTES) and quinine aptamer (Apt) were employed as dual functional monomers, and Qn was the template molecule. Near-infrared carbon dots (RCDs) were used as fluorescence signal probe, and effectively avoided the interference of fluorescence emitted by Qn.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!