AI Article Synopsis
- * The cells were reprogrammed using Sendai virus and showed characteristics of pluripotent stem cells, indicating they can become various cell types.
- * This new human induced pluripotent stem cell (hiPSC) line can be used to study the disease mechanisms of Moyamoya disease in the lab.
Article Abstract
Peripheral blood mononuclear cells (PBMCs) were collected from 55-year old male patient with a confirmed diagnosis of hemorrhagic Moyamoya disease (MMD). PBMCs were reprogrammed using Sendai virus particles delivering the four Yamanaka factors. A footprint-free hiPSC line was characterized by the expression of pluripotency markers and a normal karyotype. These cells were able to give rise to Embryoid Bodies and to a progeny of differentiated cells belonging to the 3 germ layers. This hiPSC line represents a suitable tool for modelling in vitro MMD disease to investigate the cellular mechanisms underlying the occurrence of this pathology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.scr.2016.11.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!