We discuss the intriguing photophysics of a giant molecular spoked wheel of π-conjugated arylene-alkynylene chromophores on the single-molecule level. This "molecular mesoscopic" structure, CH, shows fast switching between the 12 identical chromophores since the fluorescence is unpolarised but only one chromophore emits at a time.
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http://dx.doi.org/10.1039/c6cc08396j | DOI Listing |
J Virol
March 2025
Bioinformatics Center, Institute for Chemical Research, Kyoto University, Uji, Japan.
Sputnik virophages are small double-stranded DNA (dsDNA) viruses that replicate only inside host amoebae infected with giant dsDNA viruses, mimiviruses. Sputnik infection affects mimivirus replication, but their molecular interaction remains poorly understood. Here, we performed a time-course transcriptome analysis of cells infected with Acanthamoeba polyphaga mimivirus (APMV; hereafter referred to as Sputnik cells) and those infected with both APMV and Sputnik 3 virophage (Sputnik cells).
View Article and Find Full Text PDFRheumatology (Oxford)
March 2025
Department of Pathology, Medical University of Vienna, Vienna, Austria.
Spatial transcriptomics enables the study of the mechanisms of disease through gene expression and pathway activity analysis in a spatial context. Originally mainly employed in oncology, the techniques developed use different methods of transcript identification, resolution (single cells vs regions), flexibility of target regions and the type of molecules that can be assessed (RNA and/or protein). Selection of regions of interest requires both knowledge of the underlying histopathological changes and limitations of the methods, like artefacts due to variation in pre-analytics, or the probes used to analyse them.
View Article and Find Full Text PDFRheumatology (Oxford)
March 2025
Rheumatology Department, Health New Zealand, Auckland, New Zealand.
The session on 'Diagnosis and Classification of Vasculitis' featured six oral presentations covering various aspects of vasculitis diagnosis and classification. The application of the Ankara criteria for IgA vasculitis in adults was evaluated, finding that while the criteria showed good sensitivity, their specificity was insufficient, suggesting the need for refinement. A clustering approach to classifying ANCA-associated vasculitis (AAV) identified five distinct clusters, which improved prediction of disease outcomes.
View Article and Find Full Text PDFRheumatology (Oxford)
March 2025
Department of General Internal Medicine, UZ Leuven, Leuven, Belgium.
The breakout session "Imaging in Disease Assessment" featured six abstracts on imaging advancements for vasculitis. Disease extent on cranial MRI and its association with visual complications in giant cell arteritis (GCA) was evaluated, introducing the Propensity for Enhancement for GCA (P EG) score to assess inflammation. Predictors of remission and relapse in chronic periaortitis were analyzed, suggesting the potential for tailored treatment approaches.
View Article and Find Full Text PDFRheumatology (Oxford)
March 2025
Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds and NIHR Leeds Biomedical Research Centre, Leeds Teaching Hospitals NHS Trust, Leeds, UK.
The search for targeted therapies and biomarkers for immune-mediated systemic vasculitis requires detailed understanding of molecular pathogenesis. Whilst candidate approaches have identified new opportunities for drug repurposing, they also miss novel approaches for targeting critical immunological or stromal pathways. On the other hand, bulk transcriptional profiling may fail to capture differences in cellular composition and, depending on the cell source profiled, miss important changes within inflamed vascular tissue.
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