AI Article Synopsis
- Viral proteases, like protease 3C from coxsackievirus B3, play a crucial role in assembling viral components for proliferation and are associated with viral myocarditis.
- The study details the process of expressing, purifying, crystallizing, and analyzing the protease 3C using X-ray diffraction techniques.
- Successful production of polycrystalline protein suitable for X-ray measurements led to identifying a new polymorph with specific unit-cell parameters, marking progress toward fully understanding the protease's structure.
Article Abstract
Viral proteases are proteolytic enzymes that orchestrate the assembly of viral components during the viral life cycle and proliferation. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis are presented of protease 3C, the main protease of an emerging enterovirus, coxsackievirus B3, that is responsible for many cases of viral myocarditis. Polycrystalline protein precipitates suitable for X-ray powder diffraction (XRPD) measurements were produced in the presence of 22-28%(w/v) PEG 4000, 0.1 M Tris-HCl, 0.2 M MgCl in a pH range from 7.0 to 8.5. A polymorph of monoclinic symmetry (space group C2, unit-cell parameters a = 77.9, b = 65.7, c = 40.6 Å, β = 115.9°) was identified via XRPD. These results are the first step towards the complete structural determination of the molecule via XRPD and a parallel demonstration of the accuracy of the method.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137464 | PMC |
| http://dx.doi.org/10.1107/S2053230X16018513 | DOI Listing |
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