Protein conjugation with PAMAM nanoparticles: Microscopic and thermodynamic analysis.

Colloids Surf B Biointerfaces

Department of Chemistry-Biochemistry and Physics, University of Québec at Trois-Rivières, C. P. 500, TR (Quebec) Canada G9A 5H7, Canada. Electronic address:

Published: February 2017

PAMAM dendrimers form strong protein conjugates that are used in drug delivery systems. We report the thermodynamic and binding analysis of polyamidoamine (PAMAM-G4) conjugation with human serum albumin (HSA), bovine serum albumin (BSA) and milk beta-lactoglobulin (b-LG) in aqueous solution at physiological pH. Hydrophobicity played a major role in PAMAM-protein interactions with more hydrophobic b-LG forming stronger polymer-protein conjugates. Thermodynamic parameters showed PAMAM-protein bindings occur via hydrophobic and H-bonding contacts for b-LG, while van der waals and H-bonding interactions prevail in HSA and BSA-polymer conjugates. The protein loading efficacy was 45-55%. PAMAM complexation induced major alterations of protein conformation. TEM images show major polymer morphological changes upon protein conjugation.

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http://dx.doi.org/10.1016/j.colsurfb.2016.11.037DOI Listing

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