AI Article Synopsis
- The cancer microenvironment is complex and varies widely, influenced by both environmental factors and the behavior of cancer cells themselves, leading to changes like increased shear stress on cancer cells due to their rapid growth.
- Researchers created a microfluidic cell culture system using esophageal cancer cells to study how these cells change when exposed to fluid flow, revealing that exposure leads to significant changes in cell markers related to their transition and growth potential.
- This microfluidic system offers a promising, low-cost way to explore how fluid dynamics in the cancer environment can impact cell behavior and response to treatment, potentially aiding in the development of better cancer therapies.
Article Abstract
Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress. We report the epithelial to hybrid epithelial/mesenchymal transition as a result of decreasing E-Cadherin and increasing N-Cadherin and vimentin expressions, higher clonogenicity and ALDH positive expression of cancer cells cultured in a dynamic microfluidic chip under laminar flow compared to the static culture condition. We also sought regulation of chemotherapeutics in cancer microenvironment towards phenotypic control of cancer cells. Such in vitro microfluidic system could potentially be used to monitor how the interstitial fluid dynamics affect cancer microenvironment and plasticity on a simple, highly controllable and inexpensive bioengineered platform.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133540 | PMC |
| http://dx.doi.org/10.1038/srep38221 | DOI Listing |
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