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Trio, a Rho Family GEF, Interacts with the Presynaptic Active Zone Proteins Piccolo and Bassoon. | LitMetric

AI Article Synopsis

  • - Synaptic vesicles fuse with the plasma membrane at the presynaptic active zone, coordinated by proteins within the cytoskeletal matrix known as the cytoskeletal active zone (CAZ).
  • - The study identifies Trio, a guanine nucleotide exchange factor, as a new binding partner for CAZ proteins Piccolo and Bassoon, which helps in regulating actin assembly and synaptic growth.
  • - Findings indicate that Trio interacts with Piccolo and Bassoon through specific protein domains, and that parts of Trio can negatively regulate these interactions, suggesting a complex role in neurotransmission and vesicle recycling.

Article Abstract

Synaptic vesicles (SVs) fuse with the plasma membrane at a precise location called the presynaptic active zone (AZ). This fusion is coordinated by proteins embedded within a cytoskeletal matrix assembled at the AZ (CAZ). In the present study, we have identified a novel binding partner for the CAZ proteins Piccolo and Bassoon. This interacting protein, Trio, is a member of the Dbl family of guanine nucleotide exchange factors (GEFs) known to regulate the dynamic assembly of actin and growth factor dependent axon guidance and synaptic growth. Trio was found to interact with the C-terminal PBH 9/10 domains of Piccolo and Bassoon via its own N-terminal Spectrin repeats, a domain that is also critical for its localization to the CAZ. Moreover, our data suggest that regions within the C-terminus of Trio negatively regulate its interactions with Piccolo/Bassoon. These findings provide a mechanism for the presynaptic targeting of Trio and support a model in which Piccolo and Bassoon play a role in regulating neurotransmission through interactions with proteins, including Trio, that modulate the dynamic assembly of F-actin during cycles of synaptic vesicle exo- and endocytosis.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5132261PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0167535PLOS

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