Methylation of promoter region of gene in colorectal cancer.

The incidence of colorectal cancer is on the increase owing to changes in daily diet. In the present study, the methylation status of caudal type homeobox transcription factor 2 () gene in lesion tissue of colorectal cancer (CRC) was investigated. Additionally, the correlation between the promoter methylation of gene, CRC and gene expression in patients with CRC and normal population was examined. Between April 2014 and May 2015 78 cases with CRC were enrolled in the study. Using methylation-specific polymerase chain reaction (PCR), the promoter methylation of CDX2 in normal tissues and colorectal tissues was examinned. Through the fluorescence quantitative PCR technique, the expression levels of gene were determined in a normal population and lesion tissue of patients with CRC. At the same time, we evaluated the levels of the gene product in the normal population and lesion tissue of patients with CRC. The results showed that the methylation rate of the promoter region of gene in normal colorectal tissue was 43.5%, whereas that in the lesion tissue of CRC was 78.5%. The result was statistically significant (P<0.05). The quantity of mRNA and protein expression of gene in colorectal and normal tissue was significantly different (P<0.05). In conclusion, the methylation of the gene promoter region was associated with risk of CRC, i.e., methylation of the promoter region of gene favors the occurrence of CRC.