Identification of phenolic secondary metabolites from Sond. (Fabaceae) and demonstration of their antioxidant activities in .

Background: Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods.

Methods: assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and assays: strains maintenance, Intracellular ROS in , Survival assay, GFP expression and Subcellular DAF-16 localization were employed to evaluate the antioxidant activity.

Results: More than forty polyphenols, including flavonoid glycosides, galloylated flavonoid glycosides, isoflavones, dihydrochalcones, procyanidins, anthocyanins, hydroxy benzoic acid derivatives, hydrolysable tannins, and traces of methylated and acetylated flavonoid derivatives were identified. Three compounds were isolated and identified from the genus for the first time, namely gallic acid, myricetin-3---L--rhamnoside and quercetin-3--L--rhamnoside. The total phenolics content of SBE was (376 mg CAE/g), followed by flavonoids (67.87 QE/g). antioxidant activity of SBE was evidenced by DPPH radical scavenging activity (IC of 9 µg/mL), FRAP ferric reducing activity (5,000 mol Fe E/mg) and ABTS peroxide inhibiting activity (1,054 mM Trolox E/mg). The tested extract was able to protect the worms against juglone induced oxidative stress, an increased survival rate (up to 41%) was recorded, when compared with the control group (11%) and attenuate the reactive oxygen species (ROS) accumulation in dose-dependent and reached up to 72% for the highest tested concentration. SBE was also able to attenuate the levels of heat shock protein (HSP) expression in dose-dependent up to 60% in the 150 µg SBE/mL group. In DAF-16 Subcellular localization SBE treated worms showed nuclear localization pattern up to 78%, while it was only 5% in the untreated control group.

Discussion: A pronounced antioxidant activity , which can be attributed to its ability to promote the nuclear translocation of DAF-16/FOXO, the main transcription factor regulating the expression of stress response genes. The remarkable antioxidant activity and correlates to SBE rich phenolic profile.