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Polyethyleneimine-coated quantum dots for miRNA delivery and its enhanced suppression in HepG2 cells. | LitMetric

AI Article Synopsis

  • Quantum dots (QDs), specifically CdSe/ZnS, were modified with polyethyleneimine (PEI) to create nonviral vectors for delivering miR-26a plasmids and for bioimaging purposes.
  • The PEI-coated QDs formed nanocomplexes with the plasmid, emitting strong red luminescence and successfully transfecting miR-26a into HepG2 liver cancer cells.
  • The study found that miR-26a transfection enhanced its expression, led to cell cycle arrest, and inhibited cell proliferation, indicating the potential of these QD-based nanoparticles for gene therapy and imaging applications.

Article Abstract

Quantum dots (QDs) have been intensively investigated for bioimaging, drug delivery, and labeling probes because of their unique optical properties. In this study, CdSe/ZnS QDs-based nonviral vectors with the dual functions of delivering miR-26a plasmid and bioimaging were formulated by capping the surface of CdSe/ZnS QDs with polyethyleneimine (PEI). The PEI-coated QDs were capable of condensing miR-26a expression vector into nanocomplexes that can emit strong red luminescence when loaded with CdSe/ZnS QDs. Further results showed that PEI-modified nanoparticles (NPs) could transfect miR-26a plasmid into HepG2 cells in vitro. Meanwhile, imaging of living cells could be achieved based on the CdSe/ZnS QDs. Further study suggested that miR-26a transfection up-regulated miR-26a expression, induced cycle arrest, and triggered proliferation inhibition in HepG2 cells. The results indicated that PEI-coated QD NPs possess the capability of bioimaging and gene delivery and could be a promising vehicle with the engineering of QD NPs for gene therapy in the future.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5117883PMC
http://dx.doi.org/10.2147/IJN.S120828DOI Listing

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