(Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant's cell-wall components. Functional analyses of the genes that encode CWDEs will help explain the mechanisms of growth and pathogenicity of . Here, we isolated and characterized a gene that encoded an endo-β-1, 4-xylanase in . The expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of was induced by xylan. The deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of
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http://dx.doi.org/10.3389/fmicb.2016.01787 | DOI Listing |
Arch Biochem Biophys
February 2025
Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India. Electronic address:
Int J Biol Macromol
December 2024
Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, National Engineering Laboratory for Industrial Enzymes, Tianjin 300457, China. Electronic address:
Xylanases are a class of glycoside hydrolases commonly used in the food, papermaking, and textile industries. However, most xylanases are rapidly inactivated under harsh industrial conditions. Here, a unique and robust GH11 xylanase, AncXyn18, was designed using an ancestral sequence reconstruction strategy, sequence analysis, structure prediction, and experimental verification.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Microbiology, University of Jhang, Jhang 35200, Pakistan.
Enzymatic hydrolysis of arabinoxylan is of cost-effective strategy to yield valuable macromolecules, e.g., xylooligosaccharides (XOS).
View Article and Find Full Text PDFBiochem Biophys Res Commun
November 2024
Faculty of Engineering, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan. Electronic address:
Lignin-carbohydrate complexes (LCCs) present a considerable hurdle to the economic utilization of lignocellulosic biomass. Glucuronoyl esterase (GE) is an LCC-degrading enzyme that catalyzes the cleavage of the cross-linkages between lignin and xylan in LCCs. Benzyl-d-glucuronate (Bn-GlcA), a commercially available substrate, is widely used to evaluate GE activity assays.
View Article and Find Full Text PDFBiosci Biotechnol Biochem
November 2024
Graduate School of Bioresources, Mie University, Tsu, Japan.
Paenibacillus xylaniclasticus strain TW1 is a promising tool for decomposing xylan-containing lignocellulosic biomass, since this strain possesses various genes encoding cellulolytic/hemicellulolytic enzymes. In this study, PxRex8A from the TW1 strain was found to be a reducing-end xylose-releasing exo-oligoxylanase of glycoside hydrolase family 8, which cleaves xylose from xylooligosaccharides of corn core xylan. In a synergistic assay, the efficient decomposition of oat spelt xylan (OSX) and beech wood xylan was exemplified in the combination of endo-β-1,4-xylanase (PxXyn11A) and PxRex8A from the TW1 strain in a molar ratio of 4:1.
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