AI Article Synopsis
- - The study investigates how 1,25-Dihydroxy vitamin D affects antitumor activity in liver cancer (hepatocellular carcinoma) by analyzing gene expressions related to vitamin D metabolism in cancerous versus normal liver tissues.
- - Researchers found that in liver cancer tissues, the key genes that activate vitamin D (VDR and CYP27B1) were significantly lower, while the gene associated with inactivating vitamin D (CYP24A1) was expressed.
- - These findings suggest that liver cancer may reduce the effectiveness of 1,25-Dihydroxy vitamin D's tumor-fighting properties, highlighting a potential mechanism of cancer evasion from vitamin D's antitumor effects.
Article Abstract
Introduction: 1,25-Dihydroxy vitamin D mediates antitumor effects in hepatocellular carcinoma.
Aim: We examined mRNA and protein expression differences in 1,25-Dihydroxy vitamin D-inactivating CYP24A1, mRNA of activating CYP27B1 enzymes, and that of VDR between human hepatocellular carcinoma and surrounding non-tumorous liver.
Methods: Snap-frozen tissues from 13 patients were studied for mRNA and protein expression of CYP24A1. Paraffin-embedded tissues from 36 patients were used to study mRNA of VDR and CYP27B1. mRNA expression was measured by RT-PCR, CYP24A1 protein was detected by immunohistochemistry.
Results: Expression of VDR and CYP27B1 was significantly lower in hepatocellular carcinoma compared with non-tumorous liver (p<0.05). The majority of the HCC samples expressed CYP24A1 mRNA, but neither of the non-tumorous liver. The gene activation was followed by CYP24A1 protein synthesis.
Conclusions: The presence of CYP24A1 mRNA and the reduced expression of VDR and CYP27B1 mRNA in human hepatocellular carcinoma samples indicate decreased bioavailability of 1,25-Dihydroxy vitamin D, providing an escape mechanism from the anti-tumor effect. Orv. Hetil., 2016, 157(48), 1910-1918.
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| http://dx.doi.org/10.1556/650.2016.30592 | DOI Listing |
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