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Influence of ozonolysis time during sugarcane pretreatment: Effects on the fiber and enzymatic saccharification. | LitMetric

AI Article Synopsis

  • The study evaluated the effects of ozonolysis, sodium hydroxide, and ultrasound treatments on sugarcane bagasse for producing cellulosic ethanol through enzymatic hydrolysis.
  • The treatment successfully removed lignin and increased cellulose content, as confirmed by infrared spectroscopy and X-ray diffraction analysis, indicating structural integrity of cellulose.
  • The best results came from OBU-60 treatment, which significantly enhanced glucose release post-hydrolysis, yielding six times more glucose compared to untreated SCB, achieving a 93% efficiency of cellulose conversion.

Article Abstract

Modifications in sugarcane bagasse (SCB) from ozonolysis (O) NaOH (B) and ultrasound (U) (OBU) treatment for cellulosic ethanol production by enzymatic hydrolysis, were evaluated when increasing the exposure time of SCB to ozone. The lignin, cellulose, and hemicellulose after treatment were quantified: lignin removal and a consequent increase in cellulose content were shown using an infrared spectroscopic technique (ATR-FTIR) and chemical characterization. X-ray diffraction analysis (XRD) proved that OBU treatment does not affect the crystalline cellulose portion and electron microscopy techniques established that the fiber region most affected by the OBU treatment was the secondary cell wall, where the greatest lignin content is located. For OBU-60 treatment the lignin content was reduced and consequently there was a significant increase in cellulose content. After enzymatic hydrolysis, this pretreated SCB released 418mgglucose/g, corresponding to six times more than untreated SCB and a yield of 93% of the cellulose available.

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Source
http://dx.doi.org/10.1016/j.biortech.2016.11.043DOI Listing

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